VDI 4253 Blatt 4:2013-02
Current
The latest, up-to-date edition.
Bioaerosols and biological agents - Determination of total cell count by fluorescence analysis after staining with DAPI
Hardcopy , PDF
German - English
02-01-2013
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Preliminary Note
Introduction
1. Scope
2. Terms and definitions
3. Method basics
4. Limits and difficulties of the method
5. Apparatus, consumables, chemicals and
solutions
6. Sample preparation
7. Quantitative detection
8. Calculation and presentation of the
Results
9. Process parameters
Annex A - Establishment of the microscope
Factor
Annex B - Count record and summary
minutes
Bibliography
Provides an evaluation method for quantification of microorganisms by microscopic particle count after staining of DNA containing structures (DNA: deoxyribonucleic acid).
| DocumentType |
Standard
|
| Pages |
27
|
| PublisherName |
Verlag des Vereins Deutscher Ingenieure
|
| Status |
Current
|
| Supersedes |
| VDI 4250 Blatt 2:2015-11 | Bioaerosols and biological agents - Environmental health assessment of bioaerosols in ambient air - Risk assessment for aerosols containing Legionella |
| VDI 4258 Blatt 1:2017-03 | Bioaerosols and biological agents - Generation of test bioaerosols - Basics and requirements for test bioaerosols |
| VDI 4257 Blatt 2:2011-09 | Bioaerosols and biological agents - Emission measurement - Sampling of bioaerosols and separation in liquids |
| VDI 4251 Blatt 3:2015-08 | Measurement of airborne microorganisms and viruses in ambient air - Plant-related dispersion modelling for bioaerosols |
| VDI 4255 Blatt 3:2016-12 | Bioaerosols and biological agents - Emission factors for poultry |
| VDI 4250 Blatt 1:2014-08 | Bioaerosols and biological agents - Risk assessment of source-related ambient air measurements in the scope of environmental health - Effects of bioaerosol pollution on human health |
| VDI 4251 Blatt 2:2015-08 | Measurement of airborne microorganisms and viruses in ambient air - Determination of area-specific background concentrations |
| VDI 4251 Blatt 4:2017-01 | Measurement of airborne microorganisms and viruses in ambient air - Determination of existing load |
| VDI 3477:2016-03 | Biological waste gas purification - Biofilters |
| VDI 4255 Blatt 2:2009-12 | Bioaerosols and biological agents - Emission sources and control measures in livestock operations - Overview |
| VDI 4252 Blatt 2:2004-06 | Measurement of airborne microorganisms and viruses in ambient air - Active sampling of bioaerosols - Separation of airborne mould on gelatine/polycarbonate filters |
| VDI 4256 Blatt 1:2010-10 | Bioaerosols and biological agents - Determination of performance characteristics - Culture-based counting methods |
| VDI 4257 Blatt 1:2013-05 | Bioaerosols and biological agents - Emission measurement - Planning and performing emission measurements |
| VDI 4253 Blatt 3:2008-08 | Measurement of airborne microorganisms and viruses in ambient air - Culture based method for the quantitative determination of bacteria in air - Method after separation in liquids |
| VDI 4251-1 : 2007 COR 2 2016 | MEASUREMENT OF AIRBORNE MICROORGANISMS AND VIRUSES IN AMBIENT AIR - PLANNING OF PLANT-RELATED AMBIENT AIR MEASUREMENTS - PLUME MEASUREMENT |
| VDI 1000:2017-02 | VDI Standardisation Work - Principles and procedures |
| EN 13098:2000 | Workplace atmosphere - Guidelines for measurement of airborne micro-organisms and endotoxin |
| ISO 16000-17:2008 | Indoor air — Part 17: Detection and enumeration of moulds — Culture-based method |
| VDI 4258 Blatt 1:2017-03 | Bioaerosols and biological agents - Generation of test bioaerosols - Basics and requirements for test bioaerosols |
| VDI 4250 Blatt 1:2014-08 | Bioaerosols and biological agents - Risk assessment of source-related ambient air measurements in the scope of environmental health - Effects of bioaerosol pollution on human health |
| DIN ISO 16000-16:2009-12 | Indoor air - Part 16: Detection and enumeration of moulds - Sampling by filtration (ISO 16000-16:2008) |
| DIN ISO 16000-17:2010-06 | Indoor air - Part 17: Detection and enumeration of moulds - Culture-based method (ISO 16000-17:2008) |
| VDI 4253 Blatt 2:2004-06 | Measurement of airborne microorganisms and viruses in ambient air - Culture based method for the determination of the concentration of mould in air - Indirect method after sampling with gelatine/polycarbonate filters |
| VDI 4254 Blatt 1:2016-09 (Draft) | Bioaerosols and biological agents - Measurement of metabolites of microorganisms - Measurement of MVOC in ambient air |
| VDI 4257 Blatt 2:2011-09 | Bioaerosols and biological agents - Emission measurement - Sampling of bioaerosols and separation in liquids |
| ISO 16000-16:2008 | Indoor air — Part 16: Detection and enumeration of moulds — Sampling by filtration |
| VDI 4255-1 : 2005 COR 2016 | BIOAEROSOLS AND BIOLOGICAL AGENTS - SOURCES OF EMISSIONS AND CONTROL MEASURES - OVERVIEW |
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