UNI CEN ISO/TS 15216-2 : 2013
Withdrawn
A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.
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MICROBIOLOGY OF FOOD AND ANIMAL FEED - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS IN FOOD USING REAL-TIME RT-PCR - PART 2: METHOD FOR QUALITATIVE DETECTION
07-23-2013
01-01-2013
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus and materials
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Test report
Annex A (normative) - Diagram of procedure
Annex B (informative) - Real-time RT-PCR mastermixes
and cycling parameters
Annex C (informative) - Real-time RT-PCR primers and
hydrolysis probes for the detection of HAV, norovirus
GI and GII and mengo virus (process control)
Annex D (informative) - Growth of mengo virus strain MC[0]
for use as a process control
Annex E (informative) - RNA extraction using the BioMerieux
NucliSens[R] system
Annex F (normative) - Composition and preparation of reagents
and buffers
Annex G (informative) - Generation of external control RNA
(EC RNA) stocks
Annex H (informative) - Typical optical plate layout
Bibliography
Provides a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces.
| DocumentType |
Standard
|
| PublisherName |
Ente Nazionale Italiano di Unificazione (UNI)
|
| Status |
Withdrawn
|
| SupersededBy |
| Standards | Relationship |
| ISO/TS 15216-2:2013 | Identical |
| CEN ISO/TS 15216-2:2013 | Identical |
| ISO 22174:2005 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO 22118:2011 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics |
| ISO 22119:2011 | Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
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