UNI CEN ISO/TS 15216-1 : 2013
Current
The latest, up-to-date edition.
MICROBIOLOGY OF FOOD AND ANIMAL FEED - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS IN FOOD USING REAL-TIME RT-PCR - PART 1: METHOD FOR QUANTIFICATION
10-10-2013
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus and materials
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Test report
Annex A (normative) - Diagram of procedure
Annex B (informative) - Real-time RT-PCR mastermixes
and cycling parameters
Annex C (informative) - Real-time RT-PCR primers and
hydrolysis probes for the detection of HAV,
norovirus GI and GII and mengo virus (process
control)
Annex D (informative) - Growth of mengo virus strain
MC[0] for use as a process control
Annex E (informative) - RNA extraction using the
BioMerieux NucliSens[R] system
Annex F (normative) - Composition and preparation of
reagents and buffers
Annex G (informative) - Generation of double-stranded
DNA (dsDNA) control stocks
Annex H (informative) - Generation of external control
RNA (EC RNA) stocks
Annex I (informative) - Typical optical plate layout
Bibliography
This part of ISO 15530 introduces terminology and key concepts and provides an overview of the ISO 15530 series.
| Committee |
CT 47
|
| DocumentType |
Technical Specification
|
| PublisherName |
Ente Nazionale Italiano di Unificazione (UNI)
|
| Status |
Current
|
| Standards | Relationship |
| CEN ISO/TS 15216-1:2013 | Identical |
| ISO/TS 15216-1:2013 | Identical |
| ISO 22174:2005 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO 22118:2011 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics |
| ISO 22119:2011 | Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
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