PD CEN ISO/TS 18867:2015
Current
The latest, up-to-date edition.
Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis
Hardcopy , PDF
English
03-31-2016
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European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principles
5 Reagents
6 Apparatus and equipment
7 Sampling
8 Procedure
9 Test report
Annex A (normative) - PCR detection and isolation of
pathogenic Y. enterocolitica
Annex B (informative) - Real-time PCR for detection
of Y. enterocolitica
Annex C (informative) - Detection and isolation of
Y. pseudotuberculosis
Annex D (informative) - Simultaneous detection of
pathogenic Y. enterocolitica and Y. pseudotuberculosis
using multiplex real-time PCR
Bibliography
Defines two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCR-based methods.
| Committee |
AW/9
|
| DocumentType |
Standard
|
| Pages |
42
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
This Technical Specification specifies two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCR-based methods. The described methods allow for the detection of the two pathogens in enrichments and allow the isolation of colonies. Y. pestis, the causative agent of bubonic and pneumonic plague harbours a variant of the ail gene as well and will be detected by the same primer/probe set as Y. pseudotuberculosis. However, Y. pestis is normally not associated with food. This Technical Specification is applicable to products for human consumption, animal feeding stuffs, and environmental samples.
| Standards | Relationship |
| ISO/TS 18867:2015 | Identical |
| CEN ISO/TS 18867:2015 | Identical |
| ISO 22174:2005 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
| ISO 6887-1:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO 20837:2006 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for sample preparation for qualitative detection |
| ISO 10273:2017 | Microbiology of the food chain — Horizontal method for the detection of pathogenic Yersinia enterocolitica |
| ISO 20838:2006 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for amplification and detection for qualitative methods |
| ISO 11133:2014 | Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media |
| ISO 22119:2011 | Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
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