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EN ISO 6887-1:2017

Current

Current

The latest, up-to-date edition.

Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017)

Published date

04-05-2017

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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Diluents
6 Apparatus
7 Sampling
8 Preparation of samples
9 Specific procedures
10 Further dilutions
Annex A (informative) - Illustrations of pooling
        and compositing procedures
Annex B (informative) - Method for sampling
        frozen test pieces or blocks
Annex C (informative) - Data showing reliability
        of test results according to size of test portions
Annex D (informative) - Verification protocol for
        pooling samples for qualitative tests
Bibliography

ISO 6887-1:2017 defines general rules for the aerobic preparation of the initial suspension and of dilutions for microbiological examinations of products intended for human or animal consumption.ISO 6887-1:2017 is applicable to the general case and other parts apply to specific groups of products as mentioned in the foreword. Some aspects might also be applicable to molecular methods where matrices can be associated with inhibition of the PCR steps and consequently affect the test result.ISO 6887-1:2017 excludes preparation of samples for both enumeration and detection test methods where preparation instructions are detailed in specific International Standards.

Committee
CEN/TC 463
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

I.S. EN ISO 4833:2003 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE ENUMERATION OF MICROORGANISMS - COLONY-COUNT TECHNIQUE AT 30 DEGREE C
BS EN 15786:2009 Animal feeding stuffs. Isolation and enumeration of Pediococcus spp.
BS EN 15784:2009 Animal feeding stuffs. Isolation and enumeration of presumptive Bacillus spp.
08/30176874 DC : DRAFT APR 2008
08/30176881 DC : DRAFT APR 2008
I.S. EN 15787:2009
I.S. EN 15788:2009
BS EN ISO 8261:2002 Milk and milk products. General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination
CEN ISO/TS 11133-2 : 2003 AMD 1 2011 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GUIDELINES ON PREPARATION AND PRODUCTION OF CULTURE MEDIA - PART 2: PRACTICAL GUIDELINES ON PERFORMANCE TESTING OF CULTURE MEDIA
DIN EN 15789:2009-12 Animal feeding stuffs - Isolation and enumeration of yeast probiotic strains
DIN EN 15788:2009-12 Animal feeding stuffs - Isolation and enumeration of Enterococcus (E. faecium) spp.
I.S. EN ISO 6579:2002 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF SALMONELLA SPP
BS EN 15789:2009 Animal feeding stuffs. Isolation and enumeration of yeast probiotic strains
NF EN 15786 : 2009
NF EN 15784 : 2009
I.S. EN 15789:2009 ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF YEAST PROBIOTIC STRAINS
I.S. EN 15785:2009
UNI EN 15788 : 2009 ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF ENTEROCOCCUS (E. FAECIUM) SPP.
I.S. EN ISO 16654:2001 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF ESCHERICHIA COLI 0 157 (ISO 16654:2001)
NF EN 15787 : 2009
BS EN 15788:2009 Animal feeding stuffs. Isolation and enumeration of Enterococcus (E. faecium) spp.
08/30176889 DC : DRAFT APR 2008
UNI EN 15789 : 2009 ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF YEAST PROBIOTIC STRAINS
NF EN 15785 : 2009
NF EN 15789 : 2009 ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF YEAST PROBIOTIC STRAINS
I.S. CEN ISO TS 11133-2:2004 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GUIDELINES ON PREPARATION AND PRODUCTION OF CULTURE MEDIA - PART 2: PRACTICAL GUIDELINES ON PERFORMANCE TESTING OF CULTURE MEDIA
UNI EN 15785 : 2009 ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF BIFIDOBACTERIUM SPP.
UNI CEN ISO/TS 11133-2 : 2011 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GUIDELINES ON PREPARATION AND PRODUCTION OF CULTURE MEDIA - PART 2: PRACTICAL GUIDELINES ON PERFORMANCE TESTING OF CULTURE MEDIA
I.S. EN ISO 6888-3:2003 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE ENUMERATION OF COAGULASE-POSITIVE STAPHYLOCOCCI (STAPHYLOCOCCUS AUREUS AND OTHER SPECIES) - PART 3: DETECTION AND MPN TECHNIQUE FOR LOW NUMBERS
UNI EN 15786 : 2009 ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF PEDIOCOCCUS SPP.
UNI EN 15784 : 2009 ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF PRESUMPTIVE BACILLUS SPP.
DIN EN 15784:2009-12 Animal feeding stuffs - Isolation and enumeration of presumptive Bacillus spp.
UNI EN 15787 : 2009 ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF LACTOBACILLUS SPP.
DIN EN 15785:2009-12 Animal feeding stuffs - Isolation and enumeration of Bifidobacterium spp.
BS EN 15787:2009 Animal feeding stuffs. Isolation and enumeration of Lactobacillus spp.
NF EN 15788 : 2009
I.S. EN 15784:2009
I.S. EN 15786:2009 ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF PEDIOCOCCUS SUPP.
BS EN ISO 6579 : 2002 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF SALMONELLA SPP
BS EN ISO 16654 : 2001 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF ESCHERICHIA COLI O157
ISO 4833:2003 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of microorganisms Colony-count technique at 30 degrees C
ISO 6579:2002 Microbiology of food and animal feeding stuffs Horizontal method for the detection of Salmonella spp.
ISO 6888-3:2003 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) Part 3: Detection and MPN technique for low numbers
ISO/TS 11133-2:2003 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing of culture media
ISO 16654:2001 Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157
EN ISO 7899-2:2000 Water quality - Detection and enumeration of intestinal enterococci - Part 2: Membrane filtration method (ISO 7899-2:2000)
EN ISO 6888-3:2003 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 3: Detection and MPN technique for low numbers (ISO 6888-3:2003)
EN 15785:2009 Animal feeding stuffs - Isolation and enumeration of Bifidobacterium spp.
EN ISO 4833:2003 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of microorganisms - Colony-count technique at 30 degrees C (ISO 4833:2003)
EN ISO 8261 : 2001 MILK AND MILK PRODUCTS - GENERAL GUIDANCE FOR THE PREPARATION OF TEST SAMPLES, INITIAL SUSPENSIONS AND DECIMAL DILUTIONS FOR MICROBIOLOGICAL EXAMINATION
DIN EN 15786:2009-12 Animal feeding stuffs - Isolation and enumeration of Pediococcus spp.
DIN EN 15787:2009-12 Animal feeding stuffs - Isolation and enumeration of Lactobacillus spp.
08/30176878 DC : DRAFT APR 2008
BS EN 15785:2009 Animal feeding stuffs. Isolation and enumeration of Bifidobacterium spp.
08/30176884 DC : DRAFT APR 2008
08/30176892 DC : DRAFT APR 2008 BS EN 15789 - ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF YEAST PROBIOTIC STRAINS
DD CEN ISO/TS 11133-2 : 2003 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GUIDELINES ON PREPARATION AND PRODUCTION OF CULTURE MEDIA - PART 2: PRACTICAL GUIDELINES ON PERFORMANCE TESTING OF CULTURE MEDIA
BS EN ISO 4833:2003 Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of microorganisms. Colony-count technique at 30°C
BS EN ISO 6888-3:2003 Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) Detections and MPN technique for low numbers
ISO 8261:2001 Milk and milk products General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination
EN 15789:2009 Animal feeding stuffs - Isolation and enumeration of yeast probiotic strains
DIN EN ISO 16654:2017-08 Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157 (ISO 16654:2001 + Amd 1:2017) (includes Amendment :2017)
EN 15784:2009 Animal feeding stuffs - Isolation and enumeration of presumptive Bacillus spp.
EN 15787:2009 Animal feeding stuffs - Isolation and enumeration of Lactobacillus spp.
EN 15788:2009 Animal feeding stuffs - Isolation and enumeration of Enterococcus (E. faecium) spp.
EN 15786:2009 Animal feeding stuffs - Isolation and enumeration of Pediococcus spp.

ISO 16140-2:2016 Microbiology of the food chain — Method validation — Part 2: Protocol for the validation of alternative (proprietary) methods against a reference method
ISO 7218:2007 Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations
ISO 11133:2014 Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
ISO 7002:1986 Agricultural food products — Layout for a standard method of sampling from a lot
ISO 18593:2004 Microbiology of food and animal feeding stuffs Horizontal methods for sampling techniques from surfaces using contact plates and swabs

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