EN ISO 16712:2006

1 Scope
2 Principle
3 Test environment
   3.1 Facilities
   3.2 Lighting
4 Reagents and materials
   4.1 Test organism
   4.2 Overlying water
5 Apparatus
6 Treatment and preparation of samples
   6.1 General
   6.2 Control or reference sediment
   6.3 Test sediment
   6.4 Preparation of sediment samples
7 Test procedures
   7.1 Preparing the exposure vessels
   7.2 Introducing the organisms
   7.3 Test conditions
   7.4 Test observations and measurements
   7.5 Expression of results
   7.6 Reburrowing capability
   7.7 Validity of the test
8 Analysis and interpretation of results
   8.1 Data analysis
   8.2 Non-contaminant factors
9 Reference toxicant
10 Precision
11 Test report
Annex A (informative) Reconstitued salt water
Annex B (normative) Maximum length of amphipod species,
                      optimal salinity and temperature
                      ranges
Annex C (informative) Precision data
Bibliography

ISO 16712:2005 specifies a method for the determination of acute toxicity to amphipods exposed over a period of 10 d to samples of contaminated marine or estuarine sediment, to chemical, industrial or municipal sludge or other solid wastes that may combine with marine or estuarine sediments, or to chemicals or preparations spiked into clean sediment.