DIN EN 13704:2002-05

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Requirements
5 Test method
  5.1 Principle
  5.2 materials and reagents
  5.3 Apparatus and glassware
  5.4 Preparation of spore test suspension and test
      solutions
  5.5 Procedure
  5.6 Calculation and expression of results
  5.7 Conclusion
  5.8 Test report
Annex A (normative) Preparation of Bacillus spore stock
        suspensions
A.1 Material and reagents
A.2 Preparation of Bacillus spore stock suspensions
Annex B (normative) Validation of dilution-neutralization
        and membrane filtration methods
B.1 Principle
B.2 Preparation of spore suspension
B.3 Preparation of product test solution
B.4 Test for validation
B.5 Validation
Annex C (informative) Preparation of Clostridium sporogenes
        spore stock suspension
C.1 Culture media and reagents
C.2 Apparatus and glassware
C.3 Preparation of regenerated media and incubation conditions
C.4 Preparation of Clostridium spore stock suspension
Annex D (informative) Neutralizers
Annex E (informative) Rinsing liquids
Annex F (informative) Example of a typical test report
Annex G (informative) Referenced strains in national collections
G.1 Bacillus subtilis
G.2 Bacillus cereus
G.3 Clostridium sporogenes
Annex H (informative) Information on the application and
        interpretation of test methods in accordance with
        European standards for chemical disinfectants and
        antiseptics

This standard is included in DIN Handbook 76. Defines a test method (phase 2/step 1) and the minimum requirements for sporicidal activity of chemical disinfectant products that form a homogeneous, physically stable preparation in hard water and that are used in food, industrial, domestic and institutional areas, excluding areas and situations where disinfection is medically indicated and excluding products used on living tissues except those for hand hygiene in the above considered areas.