CEN ISO/TS 6579-2:2012
Current
The latest, up-to-date edition.
Microbiology of food and animal feed - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 2: Enumeration by a miniaturized most probable number technique (ISO/TS 6579-2:2012)
11-01-2012
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Culture media and sera
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Test report
Annex A (informative) - Composition and preparation
of culture media and reagents
Annex B (informative) - Diagram of procedure
Bibliography
ISO/TS 6579-2 gives a method for the enumeration of Salmonella spp. present in: products intended for human consumption and for the feeding of animals; environmental samples in the area of food production and food handling; animal faeces; and environmental samples from the primary production stage by calculation of the most probable number (MPN).
The method is based on miniaturization of the dilution, pre-enrichment and selective enrichment steps. The selective enrichment medium, modified semi-solid Rappaport?Vassiliadis (MSRV), is intended for the detection of motile salmonellae and is not appropriate for the detection of non-motile salmonellae.
It is possible that the method is less appropriate to enumerate Salmonella ser. Typhi and Salmonella ser. Paratyphi.
The method is not appropriate for the enumeration of Salmonella spp. in (very) low contaminated samples (
| Committee |
CEN/TC 463
|
| DocumentType |
Technical Specification
|
| PublisherName |
Comite Europeen de Normalisation
|
| Status |
Current
|
| Standards | Relationship |
| UNI CEN ISO/TS 6579-2 : 2013 | Identical |
| DIN CEN ISO/TS 6579-2;DIN SPEC 10052:2013-08 | Identical |
| XP CEN ISO/TS 6579-2 : 2013 XP | Identical |
| PD CEN ISO/TS 6579-2:2012 | Identical |
| ISO/TS 6579-2:2012 | Identical |
| S.R. CEN ISO/TS 6579-2:2012 | Identical |
| DIN SPEC 10052 : 2013 | Identical |
| NEN NPR CEN ISO/TS 6579-2 : 2012 | Identical |
| ISO 6579:2002 | Microbiology of food and animal feeding stuffs Horizontal method for the detection of Salmonella spp. |
| ISO/TS 11133-1:2009 | Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO/TS 11133-2:2003 | Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing of culture media |
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