BS ISO 7251:2005
Withdrawn
A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.
Microbiology of food and animal feeding stuffs. Horizontal method for the detection and enumeration of presumptive Escherichia coli. Most probable number technique
Hardcopy , PDF
10-20-2023
English
05-05-2005
Important note : All end users must be registered with an Account prior to user licenses being assigned.
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Detection method
4.2 Enumeration method
5 Diluent, culture media and reagent
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Detection method
9.2 Enumeration method
10 Expression of results
10.1 Detection method
10.2 Enumeration method
11 Test report
Annex A (normative) MPN table
Bibliography
Provides general guidelines for the detection and enumeration of presumptive Escherichia coli by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at 37 degree C, then at 44 degree C.
| Committee |
AW/9
|
| DevelopmentNote |
Supersedes BS 5763-8(1994). (05/2005)
|
| DocumentType |
Standard
|
| Pages |
22
|
| PublisherName |
British Standards Institution
|
| Status |
Withdrawn
|
| Supersedes |
This International Standard gives general guidelines for the detection and enumeration of presumptive Escherichia coli by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at 37°C, then at 44°C. This International Standard is applicable to
-
products intended for human consumption and the feeding of animals, and
-
environmental samples in the area of food production and food handling.
WARNING — Some pathogenic strains of Escherichia coli do not grow at 44°C.
A limitation of the applicability of this International Standard is imposed by the susceptibility of the method to a large degree of variability (see Clause10).
| Standards | Relationship |
| ISO 7251:2005 | Identical |
| ISO/TS 11133-1:2009 | Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory |
| ISO 6887-1:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO/TS 11133-2:2003 | Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing of culture media |
| ISO 8261:2001 | Milk and milk products General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination |
| ISO 6887-4:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of miscellaneous products |
| ISO 6887-3:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products |
| ISO 6887-2:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 2: Specific rules for the preparation of meat and meat products |
Access your standards online with a subscription
Features
-
Simple online access to standards, technical information and regulations.
-
Critical updates of standards and customisable alerts and notifications.
-
Multi-user online standards collection: secure, flexible and cost effective.