BS ISO 21527-2:2008

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Diluent and culture medium
6 Apparatus and glassware
7 Sampling
8 Preparation of the test sample
9 Procedure
10 Expression of results and confidence limits
11 Test report
Annex A (informative) - Examples of water activity according
                        to matrices
Bibliography

Describes a horizontal method for the enumeration of viable osmophilic yeasts and xerophilic moulds in products intended for human consumption or feeding of animals, having a water activity less than or equal to 0,95 (dry fruits, cakes, jams, dried meat, salted fish, grains, cereals and cereals products, flours, nuts, spices and condiments, etc.), by means of the colony count technique at 25 degrees C plus or minus 1 degrees C.

This part of ISO21527 specifies a horizontal method for the enumeration of viable osmophilic yeasts and xerophilic moulds in products intended for human consumption or feeding of animals that have a water activity less than or equal to 0,95 (dry fruits, cakes, jams, dried meat, salted fish, grains, cereals and cereal products, flours, nuts, spices and condiments, etc. [AnnexA]), by means of the colony count technique at 25°C±1°C (Reference [3]).

This part of ISO21527 does not apply to dehydrated products with water activity less than or equal to 0,60 (dehydrated cereals, oleaginous products, spices, leguminous plants, seeds, powders for instant drinks, dry products for domestic animals, etc.) and does not allow the enumeration of mould spores (Reference [3]). Neither the identification of fungal flora nor the examination of foods for mycotoxins lie within the scope of this part of ISO21527. The method specified in this part of ISO21527 is not suitable for enumeration of halophilic xerophilic fungi (i.e. Polypaecilum pisce, Basipetospora halophila) such as may be found in dried fish.