BS ISO 16303:2013

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
6 Reagents, test organisms, and materials
7 Apparatus
8 Treatment and preparation of samples
9 Test procedure
10 Expression of results
11 Analysis and interpretation of results
12 Reference substance
13 Test report
Annex A (informative) - Description of Hyalella azteca
Annex B (informative) - Culturing Hyalella azteca
Annex C (informative) - Sources of Hyalella azteca
Annex D (normative) - Procedure for preparing YCT
Annex E (informative) - 42-day sediment reproduction test
Annex F (informative) - 14-day water-only
        survival-and-growth test
Annex G (informative) - Performance data
Bibliography

Describes a method for the determination of toxicity to young Hyalella azteca in whole sediment based on survival and growth inhibition after 14 d and/or 28 d.

This International Standard specifies a method for the determination of toxicity to young Hyalella azteca in whole sediment based on survival and growth inhibition after 14d and/or 28d. The method is applicable to samples of contaminated whole fresh water sediment, chemical, industrial, or municipal sludge, or other solid wastes that may combine with fresh water sediments, and chemicals or preparations spiked into clean sediment. This International Standard is applicable to the testing of sediment samples from the fresh water environment. Hyalella azteca can be used in the testing of brackish waters up to a maximum of15‰, with careful acclimation. This International Standard is not applicable to the testing of sediment samples from the marine and estuarine environment with a salinity of>15‰. This method is a 14d and/or 28d survival-and-growth test applicable to the sediment sample types described above.