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BS EN ISO 11268-1:2015

Current

Current

The latest, up-to-date edition.

Soil quality. Effects of pollutants on earthworms Determination of acute toxicity to Eisenia fetida/Eisenia andrei

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

08-31-2015

US$257.86
Excluding Tax where applicable

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and material
6 Apparatus
7 Procedure
8 Calculation and expression of results
9 Validity of the test
10 Statistical analysis
11 Test report
Annex A (informative) - Determination of the acute toxicity
        of chemicals (in particular pesticides) on earthworms
        under tropical test conditions
Annex B (informative) - Culturing of Eisenia fetida and
        Eisenia andrei
Annex C (informative) - Determination of water holding
        capacity of artificial soil
Annex D (informative) - Background information on the
        acute effect of boric acid on earthworms
Bibliography

Describes one of the methods for evaluating the habitat function of soils and determining the acute toxicity of soil contaminants and chemicals to Eisenia fetida/Eisenia andrei by dermal and alimentary uptake.

Committee
EH/4
DevelopmentNote
Supersedes BS 7755-4.2.1(1994) & 11/30213866 DC. (08/2015)
DocumentType
Standard
Pages
30
PublisherName
British Standards Institution
Status
Current
Supersedes

This part of ISO 11268 specifies one of the methods for evaluating the habitat function of soils and determining the acute toxicity of soil contaminants and chemicals to Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. It is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials.

Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects on survival are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil comparable to the soil sample to be tested (reference soil) or a standard soil (e.g. artificial soil).

Information is provided on how to use this method for testing chemicals under temperate as well as under tropical conditions.

The method is not applicable to volatile substances, i.e. substances for which H (Henry’s constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.

This method does not take into account the possible degradation of the substances or contaminants during the test.

Standards Relationship
EN ISO 11268-1:2015 Identical
ISO 11268-1:2012 Identical

ISO 10381-6:2009 Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 17616:2008 Soil quality Guidance on the choice and evaluation of bioassays for ecotoxicological characterization of soils and soil materials
ISO 11277:2009 Soil quality Determination of particle size distribution in mineral soil material Method by sieving and sedimentation
ISO 15799:2003 Soil quality Guidance on the ecotoxicological characterization of soils and soil materials
ISO 10390:2005 Soil quality Determination of pH
ISO 10694:1995 Soil quality — Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 15176:2002 Soil quality Characterization of excavated soil and other soil materials intended for re-use
ISO 11260:1994 Soil quality Determination of effective cation exchange capacity and base saturation level using barium chloride solution

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