ASTM E 2694 : 2011
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
Hardcopy , PDF
11-11-2014
English
08-01-2011
Important note : All end users must be registered with an Account prior to user licenses being assigned.| Committee |
E 34
|
| DocumentType |
Test Method
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| Pages |
8
|
| PublisherName |
American Society for Testing and Materials
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| Status |
Superseded
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| SupersededBy | |
| Supersedes |
1.1 The method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with microorganisms found in water-miscible metalworking fluids (MWF).
1.2 The ATP is measured using a bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the samples. The light is produced and measured quantitatively as relative light units (RLU) which are converted by comparison with an ATP standard and computation to pg ATP/mL.
1.3 This method is equally suitable for use in the laboratory or field.
1.4 The method detects ATP concentrations in the range of 4.0 pg ATP/mL to 400,000 pg ATP/mL.
1.5 Providing interferences can be overcome, bioluminescence is a reliable and proven method for qualifying and quantifying ATP. The method does not differentiate between ATP from different sources, for example, from different types of microorganisms, such as bacteria and fungi.
1.6 The values stated in SI are to be regarded as standard.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
| ASTM D 7687 : 2017 | Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration |
| ASTM E 2148 : 2016 | Standard Guide for Using Documents Related to Metalworking or Metal Removal Fluid Health and Safety |
| ASTM E 2889 : 2012 : R2017 | Standard Practice for Control of Respiratory Hazards in the Metal Removal Fluid Environment |
| ASTM E 2275 : 2014 | Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance |
| ASTM D 4012 : 2015 | Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water |
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