AS/NZS 1766.2.16.1:1998

1 - AS/NZS 1766.2.16.1:1998 FOOD MICROBIOLOGY - EXAMINATION FOR SPECIFIC ORGANISMS-FOOD AND ANIMAL FEEDING STUFFS-HORIZONTAL ....
1 - PREFACE
2 - CONTENTS
3 - 1 Scope
3 - 2 Normative references
3 - 3 Definitions
3 - 3.1 Listeria monocytogenes:
3 - 3.2 detection of Listeria monocytogenes:
4 - 4 Principle
4 - 4.1 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half Fraser broth
4 - 4.2 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth)
4 - 4.3 Plating out and identification
4 - 4.4 Confirmation
4 - 5 Culture media and reagents
5 - 6 Apparatus and glassware
5 - 6.1 Apparatus for dry sterilization (oven) or wet sterilization (autoclave)
5 - 6.2 Drying cabinet or incubator,
5 - 6.3 Incubators,
5 - 6.4 Water bath,
5 - 6.5 Loops,
5 - 6.6 pH-meter,
5 - 6.7 Test tubes or flasks,
5 - 6.8 Measuring cylinders,
5 - 6.9 Total-delivery graduated pipettes,
5 - 6.10 Petri dishes,
5 - 6.11 Jars,
5 - 6.12 Gas mixture
5 - 6.13 Equipment for the Henry illumination test (optional).
5 - 6.14 Microscope,
5 - 7 Sampling
5 - 8 Preparation of test sample
5 - 9 Procedure
5 - 9.1 Test portion and initial suspension
6 - 9.2 Primary enrichment
6 - 9.3 Secondary enrichment
6 - 9.4 Plating out and identification
6 - 9.5 Confirmation of Listeria spp.
6 - 9.5.1 Selection of colonies for confirmation
7 - 9.5.2 Catalase reaction
7 - 9.5.3 Gram staining
7 - 9.5.4 Motility test (if necessary)
7 - 9.6 Confirmation of L. monocytogenes
7 - 9.6.1 Haemolysis test
7 - 9.6.2 Carbohydrate utilization
7 - 9.6.3 CAMP test
8 - 9.7 Interpretation of morphological and physiological properties and of the biochemical reactions
8 - 9.8 Definitive confirmation
9 - 9.9 Control cultures
9 - 10 Expression of results
9 - 11 Test report
10 - Annex A - Diagram of procedure
11 - Annex B - Composition and preparation of culture media and reagents
11 - B.1 Selective primary enrichment medium: Halt Fraser broth
12 - B.2 Selective secondary enrichment medium: Fraser broth
13 - B.3 First selective plating-out medium: Oxford agar
13 - B.4 Second selective plating-out medium: PALCAM agar
14 - B.5 Solid culture medium: Tryptone soya yeast extract agar (TSYEA)
15 - B.6 Liquid culture medium: Tryptone soya yeast extract broth (TSYEB)
15 - B.7 Sheep blood agar
15 - B.8 Carbohydrate utilization broth (rhamnose and xylose)
16 - B.9 Motility agar
16 - B.10 CAMP (Christie, Atkins, Munch-Petersen) medium and test strains
16 - B.11 Hydrogen peroxide solution
17 - B.12 Phosphate-buffered saline (PBS)
18 - Annex C - HENRY ILLUMINATION TEST
19 - ANNEX ZZ - ADDITIONAL REQUIREMENTS TO ISO 11290-1:1996 FOR AUSTRALIAN/NEW ZEALAND CONDITIONS
19 - ZZ1 SCOPE
19 - ZZ2 ADDITIONAL REQUIREMENTS

This part specifies a horizontal method for the detection of Listeria monocytogenes.

This part of ISO 11290 specifies a horizontal method for the detection of Listeria monocytogenes.Subject to the limitations discussed in the introduction, this part of ISO 11290 is applicable to products intended for human consumption or animal feeding.

First published as AS/NZS 1766.2.16.1:1998.