EN ISO 16212:2017

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principles
5 Diluents, neutralizers and culture media
6 Apparatus and glassware
7 Strain of microorganisms
8 Handling of cosmetic products and laboratory samples
9 Procedure
10 Counting of colonies (plate counts and membrane
   filtration methods)
11 Expression of results
12 Neutralization of the antifungicidal properties of the
   product
13 Test report
Annex A (informative) - Other neutralizing diluents
Annex B (informative) - Other diluents
Annex C (informative) - Other culture media
Annex D (informative) - Neutralizers of antifungicidal
        activity of preservatives and rinsing liquids
Bibliography

ISO 16212:2017 gives general guidelines for enumeration of yeast and mould present in cosmetics by counting the colonies on selective agar medium after aerobic incubation.In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which ISO 16212:2017 is applicable. Products considered to present a low microbiological risk (see ISO 29621) include those with low water activity or extreme pH values, hydro-alcoholic products, etc.Because of the large variety of cosmetic products within this field of application, this method might not be suited to some products in every detail (e.g. certain water-immiscible products). Other methods (e.g. automated) can be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.Yeast enumerated can be identified using suitable identification tests, for example, tests described in the standards listed in the Bibliography. Mould enumerated can be identified by other appropriate methods, if necessary.