S.R. CEN ISO/TS 15216-1:2013
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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MICROBIOLOGY OF FOOD AND ANIMAL FEED - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS IN FOOD USING REAL-TIME RT-PCR - PART 1: METHOD FOR QUANTIFICATION (ISO/TS 15216-1:2013, CORRECTED VERSION 2013-05-01)
Hardcopy , PDF
16-04-2017
English
01-01-2013
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus and materials
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Test report
Annex A (normative) - Diagram of procedure
Annex B (informative) - Real-time RT-PCR mastermixes
and cycling parameters
Annex C (informative) - Real-time RT-PCR primers and
hydrolysis probes for the detection of HAV,
norovirus GI and GII and mengo virus (process
control)
Annex D (informative) - Growth of mengo virus strain
MC[0] for use as a process control
Annex E (informative) - RNA extraction using the
BioMerieux NucliSens[R] system
Annex F (normative) - Composition and preparation of
reagents and buffers
Annex G (informative) - Generation of double-stranded
DNA (dsDNA) control stocks
Annex H (informative) - Generation of external control
RNA (EC RNA) stocks
Annex I (informative) - Typical optical plate layout
Bibliography
Defines a method for quantification of levels of HAV and NoV genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs or food surfaces.
| DocumentType |
Standard
|
| Pages |
47
|
| PublisherName |
National Standards Authority of Ireland
|
| Status |
Superseded
|
| SupersededBy |
| Standards | Relationship |
| CEN ISO/TS 15216-1:2013 | Identical |
| ISO/TS 15216-1:2013 | Identical |
| ISO 22174:2005 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO 22118:2011 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics |
| ISO 22119:2011 | Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
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