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UNI CEN ISO/TS 13136 : 2013

Current

Current

The latest, up-to-date edition.

MICROBIOLOGY OF FOOD AND ANIMAL FEED - REAL-TIME POLYMERASE CHAIN REACTION (PCR)-BASED METHOD FOR THE DETECTION OF FOODBORNE PATHOGENS - HORIZONTAL METHOD FOR THE DETECTION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC) AND THE DETERMINATION OF O157, O111, O26, O103 AND O145 SEROGROUPS

Published date

14-02-2013

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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Diluents, culture media and reagents
6 Equipment
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Performance data
Annex A (normative) - Flow diagram of the
        screening procedure
Annex B (normative) - Flow diagram of the
        isolation and confirmation procedure
Annex C (informative) - Identification of Shiga
        toxin-producing Escherichia coli
        (STEC) by multiplex PCR amplification
        of virulence genes and detection of PCR
        products by agarose gel electrophoresis
Annex D (informative) - Internal amplification control
Annex E (informative) - Primers and probes for the
        PCR assays
Annex F (normative) - Isolation of STEC strains
Bibliography

Specifies the identification of Shiga toxin-producing Escherichia coli (STEC) by means of the detection of the following genes: a) the major virulence genes of STEC, stx and eae; b) the genes associated with the serogroups O157, O111, O26, O103, and O145.

Committee
CT 3
DocumentType
Standard
PublisherName
Ente Nazionale Italiano di Unificazione (UNI)
Status
Current

Standards Relationship
CEN ISO/TS 13136:2012 Identical
ISO/TS 13136:2012 Identical

ISO 22174:2005 Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions
ISO/TS 19036:2006 Microbiology of food and animal feeding stuffs Guidelines for the estimation of measurement uncertainty for quantitative determinations
ISO 7218:2007 Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations
ISO 7550:1985 Laboratory glassware — Disposable micropipettes
ISO 20837:2006 Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for sample preparation for qualitative detection
ISO 20838:2006 Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for amplification and detection for qualitative methods
ISO 16140:2003 Microbiology of food and animal feeding stuffs Protocol for the validation of alternative methods
ISO 16654:2001 Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157

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