I.S. EN ISO 22959:2009
Current
The latest, up-to-date edition.
ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS BY ON-LINE DONOR-ACCEPTOR COMPLEX CHROMATOGRAPHY AND HPLC WITH FLUORESCENCE DETECTION
Hardcopy , PDF
English
01-01-2009
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents, materials and standards
6 Apparatus
7 Sampling and preparation of the test sample
8 Sample preparation
9 Procedure
9.1 HPLC analysis
9.2 Identification of PAHs
10 Calculation of individual PAHs
11 Method validation data
11.1 Accuracy
11.2 Within-laboratory precision
11.3 Recovery
11.4 Dynamic range
11.5 Limit of quantification
12 Precision
12.1 International collaborative trial
12.2 Repeatability
12.3 Reproducibility
13 Test report
Annex A (informative) - Example of the individual parts
of an HPLC system
Annex B (informative) - Example of the operating conditions
of the pumps of the HPLC system
Annex C (informative) - Example of the operating conditions
of the column thermostat and the detector of the
HPLC system
Annex D (informative) - Example of the autosampler programme
of the HPLC system
Annex E (informative) - Tubing connections of the HPLC system
Annex F (informative) - Chromatogram of a standard calibration
sample
Annex G (informative) - Determination precision for a sunflower
oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex H (informative) - Determination precision for an olive
oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex I (informative) - Determination precision for a soybean
oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex J (informative) - Determination precision for a coconut
oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex K (informative) - Interlaboratory test
Bibliography
Describes a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils.
| DocumentType |
Standard
|
| Pages |
36
|
| PublisherName |
National Standards Authority of Ireland
|
| Status |
Current
|
| Standards | Relationship |
| ISO 22959:2009 | Identical |
| EN ISO 22959:2009 | Identical |
| ISO 5725-1:1994 | Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions |
| ISO 5555:2001 | Animal and vegetable fats and oils — Sampling |
| ISO 1042:1998 | Laboratory glassware — One-mark volumetric flasks |
| ISO 5725-2:1994 | Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method |
| ISO 661:2003 | Animal and vegetable fats and oils — Preparation of test sample |
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