I.S. EN 16418:2014
Current
The latest, up-to-date edition.
PAPER AND BOARD - DETERMINATION OF THE CYTOTOXICITY OF AQUEOUS EXTRACTS USING A METABOLICALLY COMPETENT HEPATOMA CELL LINE (HEPG[2])
Hardcopy , PDF
English
01-01-2014
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Cell line
7 Food simulants used for testing
8 Cleaning laboratory glassware
9 Equipment
10 Preparation of specimens
11 Cytotoxicity assessment
12 Expression of the results
13 Interpretation of the results
14 Precision
15 Test report
Annex A (informative) - 96-well plates configuration
Annex B (informative) - RNA Synthesis rate
inhibition cytotoxicity test work flow
Annex C (informative) - Validation of the two
methods (option A and B)
Bibliography
Defines a test method for the laboratory assessment of the potential cytotoxic effect of paper and board intended to come into contact with foodstuffs using specifically the HepG2 cell line.
| DocumentType |
Standard
|
| Pages |
24
|
| PublisherName |
National Standards Authority of Ireland
|
| Status |
Current
|
| Standards | Relationship |
| EN 16418:2014 | Identical |
| EN 645:1993 | Paper and board intended to come into contact with foodstuffs - Preparation of a cold water extract |
| EN 15845:2010 | Paper and board - Determination of the cytotoxicity of aqueous extracts |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| EN ISO 3696:1995 | Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) |
| EN 647:1993 | Paper and board intended to come into contact with foodstuffs - Preparation of a hot water extract |
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