EN ISO 14183:2008

1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparation of test sample
8 Procedure
   8.1 Preparation of quality control sample
   8.2 Extraction
   8.3 HPLC analysis
   8.4 Determination
9 HPLC confirmation
   9.1 General
   9.2 Post-column derivatization with DMAB
   9.3 Hexane extraction
10 Calculation of results
   10.1 General
   10.2 Monensin
   10.3 Salinomycin
   10.4 Narasin
   10.5 Interpretation of confirmation data
11 Precision
   11.1 Interlaboratory test
   11.2 Repeatability
   11.3 Reproducibility
   11.4 Limit of quantitation
12 Test report
Annex A (informative) - Results of interlaboratory test
Bibliography

ISO 14183:2005 specifies a high-performance liquid chromatographic (HPLC) method for the determination of the monensin, narasin and salinomycin contents of animal feeding stuffs, supplements (dry and liquid) and mineral premixtures. The method is not applicable to drug premixes (pharmaceutical products). Lasalocid and semduramicin cannot be determined by this method.The limit of quantitation is approximately 1 mg/kg, 2 mg/kg and 2 mg/kg for monensin, salinomycin and narasin, respectively. A lower limit of quantitation can be achievable but this is to be validated by the user.