CEN/TR 15214-2:2006

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus
6 Sampling and hazards
   6.1 Introduction
   6.2 General
   6.3 Storage
   6.4 Handling
7 Reagents, diluents and culture media
   7.1 Tryptone salt diluent
   7.2 Special diluent (SD)
   7.3 Demineralized or distilled water
   7.4 Culture medium: MUG/EC medium
8 Procedure
   8.1 Sample preparation
   8.2 Analysis
9 Expression of results
   9.1 Determination of the characteristic number
   9.2 Calculation of the MPN and its confidence interval
10 Test report
11 Performance data
Annex A (informative) MPN Statistical Table
6 dilutions (1/20 to 1/2000000)/16 wells seeded per dilution
Annex B (informative) Synthetic sea salt
Annex C (informative) Performance data of the method
Annex D (normative) Quality criteria for the manufacturing of
        the medium in microtitre plates (E.coli)
Bibliography

This part of the CEN Technical Report describes a miniaturized most probable number (MPN) method for the quantitative detection of Escherichia coli in sludges, soils, soil improvers, growing media and biowastes. It is suitable to evaluate the log reduction of E. coli through treatment as well as the quality of the end product.This method is convenient for material with dry residue of more than 10 %. For materials with dry residue less than 20 %, the procedure specified in CEN/TR 15214-1 will be used.