ASTM D 8243 : 2019
Current
Current
The latest, up-to-date edition.
Standard Test Method for Determination of APS Reductase to Estimate Sulfate Reducing Bacterial Bioburdens in Water – Enzyme-Linked Immunosorbent Assay Method
Available format(s)
Hardcopy , PDF
Language(s)
English
Published date
01-01-2019
Important note : All end users must be registered with an Account prior to user licenses being assigned.1.1 This test method provides a protocol for using enzyme-linked immunosorbent assay (ELISA) technology to test water samples for the enzyme adenosine 5’-phosphosulfate reductase (APSr) concentration.
| Committee |
D 19
|
| DocumentType |
Test Method
|
| Pages |
9
|
| PublisherName |
American Society for Testing and Materials
|
| Status |
Current
|
1.1This test method provides a protocol for using enzyme-linked immunosorbent assay (ELISA) technology to test water samples for the enzyme adenosine 5’-phosphosulfate reductase (APSr) concentration.
1.1.1APSr is present in all known sulfate reducing protists (SRP – sulfate reducing bacteria – SRB – and sulfate reducing archaea – SRA).
1.1.2As reported in U.S. Patent 4,999,286, APS reductase concentration can be used as a surrogate parameter for estimating SRA bioburdens (Appendix X1 compares results from Test Methods D8243, D4412, and quantitative polymerase chain reaction – qPCR – testing).
1.2This test method has been validated in tap water, oilfield produced water (salinities ranging from 100 g L-1 to 600 g L-1), and fuel-associated water (commonly referred to as bottoms-water).
1.3This test method detects APS reductase semi-quantitatively in the range of 0.001M to 0.1M – correlating to 102 SRP/mL to 106 SRP/mL.
1.3.1As described in Appendix X2 test method sensitivity can be increased 10-fold to 100-fold. However, the precision statistics provided in X apply only to 10-mL specimens.
1.4The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Some specific hazards statements are given in Section 9 on Hazards.
1.6This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
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| ASTM E 1847 : 1996 : R2003 | Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines |
| ASTM D 6499 : 2018 | Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products |
| ASTM E 1601 : 2010 | Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method |
| ASTM D 6300 : 2014 : REV A | Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants |
| ASTM D 4412 : 1984 : R2009 | Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits |
| ASTM E 691 : 2022 | Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method |
| ASTM D 6300 : 2015 | Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants |
| ASTM D 6300 : 1999 | Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants |
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| ASTM E 1601 : 2012 | Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method |
| ASTM D 1129 : 2004 | Standard Terminology Relating to Water |
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| ASTM D 6499 : 2016 | Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products |
| ASTM D 6300 : 2013 : REV A | Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants |
| ASTM D 7464 : 2014 | Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing |
| ASTM E 691 : 2021 | Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method |
| ASTM D 6499 : 2007 | Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products |
| ASTM D 6300 : 2008 | Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants |
| ASTM D 6300 : 2003 | Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants |
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| ASTM D 6300 : 2002 | Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants |
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