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ASTM D 4012 : 1981 : R2002

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by

Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water

Available format(s)

Hardcopy , PDF

Superseded date

11-11-2014

Language(s)

English

Published date

29-05-1981

£50.68
Excluding VAT

Committee
D 19
DocumentType
Test Method
Pages
4
ProductNote
Reconfirmed 2002
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

1.1 This test method covers the measurement of adenosine triphosphate (ATP) in microorganisms in concentrations normally found in laboratory cultures, waters, wastewaters, and in plankton and periphyton samples from waters.

1.2 Knowledge of the concentration of ATP can be related to viable biomass or metabolic activity, or by utilizing an average concentration (or amount) of ATP per cell, an estimated count of microorganisms can be obtained in the case of unispecies cultures.

1.3 This test method offers a high degree of sensitivity, rapidity, accuracy, and reproducibility. However, extreme care must be taken at each step in the analysis to ensure meaningful and reliable results.

1.4 The analyst should be aware that the precision statement pertains only to determinations in reagent water and not necessarily in the matrix being tested.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM D 7847 : 2017 Standard Guide for Interlaboratory Studies for Microbiological Test Methods
ASTM D 6469 : 2017 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 7687 : 2017 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
ASTM D 7463 : 2018 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
ASTM E 2694 : 2016 Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
ASTM E 1326 : 2015 : REV A Standard Guide for Evaluating Non-culture Microbiological Tests

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