NBN EN ISO 22174 : 2005
Current
The latest, up-to-date edition.
MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
12-01-2013
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Preliminary microbial enrichment
4.3 Nucleic acid preparation
4.4 PCR amplification
4.5 Detection and confirmation of PCR products
5 Test material
6 General laboratory requirements
6.1 General
6.2 Personnel
6.3 Laboratory setup
6.4 Waste management
7 Reagents
8 Apparatus and equipment
8.1 General
8.2 Special considerations
9 Procedure
9.1 Sample preparation
9.2 Amplification
9.3 Control reaction
9.4 Confirmation of PCR results
10 Evaluation
11 Test report
Bibliography
Describes the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). Applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne pathogens using the polymerase chain reaction (PCR).
| DocumentType |
Standard
|
| PublisherName |
Belgian Standards
|
| Status |
Current
|
| Standards | Relationship |
| ISO 22174:2005 | Identical |
| BS EN ISO 22174:2005 | Identical |
| UNE-EN ISO 22174:2005 | Identical |
| UNI EN ISO 22174 : 2005 | Identical |
| EN ISO 22174:2005 | Identical |
| DIN EN ISO 22174:2005-05 | Identical |
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