EN ISO 6341:2012

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test environment
6 Reagents, test organisms and media
7 Apparatus and materials
8 Treatment and preparation of samples
9 Procedure
10 Interpretation and validity of the results
11 Expression of results
12 Test report
Annex A (informative) - Preparation of the Elendt M4 medium
Annex B (informative) - Example of graphical determination of
        the inhibition of mobility of Daphnia magna by an
        effluent or stock solution of a substance at a
        concentration of 1000 mg/l
Annex C (informative) - General recommendations for stock
        culturing
Annex D (informative) - Culturing of Daphnia magna for
        production of dormant eggs
Annex E (informative) - Precision data
Annex F (informative) - Dilution level D - Preparation of
        the dilution series for the determination of the LID
Bibliography

ISO 6341.2012 specifies a method for the determination of the acute toxicity to Daphnia magna Straus (Cladocera, Crustacea).This method is applicable to: chemical substances which are soluble under the conditions of the test, or can be maintained as a stable suspension or dispersion under the conditions of the test; industrial or sewage effluents; treated or untreated waste water; aqueous extracts and leachates; fresh water (surface and ground water); eluates of fresh water sediment; pore water of fresh water sediment.