CEN ISO/TS 15216-1:2013

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus and materials
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Test report
Annex A (normative) - Diagram of procedure
Annex B (informative) - Real-time RT-PCR mastermixes
        and cycling parameters
Annex C (informative) - Real-time RT-PCR primers and
        hydrolysis probes for the detection of HAV,
        norovirus GI and GII and mengo virus (process
        control)
Annex D (informative) - Growth of mengo virus strain
        MC[0] for use as a process control
Annex E (informative) - RNA extraction using the
        BioMerieux NucliSens[R] system
Annex F (normative) - Composition and preparation of
        reagents and buffers
Annex G (informative) - Generation of double-stranded
        DNA (dsDNA) control stocks
Annex H (informative) - Generation of external control
        RNA (EC RNA) stocks
Annex I (informative) - Typical optical plate layout
Bibliography

ISO/TS 15216-1:2013 describes a method for quantification of levels of HAV and NoV genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.