BS ISO 22196:2011

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Materials
5 Apparatus
6 Sterilization of apparatus and storage of
   stock cultures
7 Procedure
8 Expression of results
9 Repeatability and reproducibility
10 Test report
Annex A (normative) - Quality of biological
        materials
Annex B (informative) - Repeatability and
        reproducibility
Bibliography

Describes a method of evaluating the antibacterial activity of antibacterial-treated plastics, and other non-porous, surfaces of products (including intermediate products).

WARNING — Handling and manipulation of microorganisms which are potentially hazardous requires a high degree of technical competence and may be subject to current national legislation and regulations. Only personnel trained in microbiological techniques should carry out such tests. Appropriate practices for disinfection, sterilization and personal hygiene must be strictly observed.

This International Standard specifies a method of evaluating the antibacterial activity of antibacterial-treated plastics, and other non-porous, surfaces of products (including intermediate products).

It is not intended to be used to evaluate the effects and propagation of bacteria on non-porous surfaces without antibacterial treatments. ISO846^[1] describes tests to evaluate the effects and propagation of bacteria on non-porous surfaces, which are different from those covered by this International Standard (seee.g. ISO846:1997, method C).

Secondary effects of antibacterial treatments, such as the prevention of biodeterioration and odour, are not covered by this International Standard, which is not intended to be used or referenced as a method to document or claim biodegradability of, for instance, plastics materials. In the case of plastics, biodegradation is covered in ISO14851^[2], ISO14852^[3] and ISO14855^[4] and related standards.

Building materials are excluded, except where they are used in the same manner as treated articles.

Antibacterial-treated textile products are excluded, even if the surfaces are coated or laminated (such products are covered by ISO20743^[5]).

Photocatalytic materials and products are excluded (such materials and products are covered by ISO27447^[6]).

The results obtained should include a reference to this International Standard and the conditions used. Results obtained with this International Standard indicate antibacterial activity under the specified experimental conditions used, and do not reflect activity under other circumstances where a variety of factors, such as temperature, humidity, different bacterial species, nutrient conditions, etc., have to be considered. A minimum diffusion of the antibacterial agents/chemicals into the test inoculum is necessary with this procedure.

It is recommended that workers consult ISO7218.