BS EN ISO 16649-3:2015
Current
The latest, up-to-date edition.
Microbiology of the food chain. Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide
Hardcopy , PDF
English
28-02-2017
Important note : All end users must be registered with an Account prior to user licenses being assigned.
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Dilution fluids and culture media
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Precision
12 Test report
Bibliography
Defines a horizontal method for the detection and enumeration of beta-glucuronidase positive Escherichia coli, by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at (37 +/- 1) degrees C, then at (44 +/- 1) degrees C.
| Committee |
AW/9
|
| DevelopmentNote |
Supersedes DD ISO/TS 16649-3 & 13/30283641 DC. (06/2015)
|
| DocumentType |
Standard
|
| Pages |
22
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
This part of ISO16649 specifies a horizontal method for the detection and enumeration of β-glucuronidase positive Escherichia coli , by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at (37±1)°C, then at (44±1)°C. This part of ISO16649 is applicable to the following: products intended for human consumption and the feeding of animals; environmental samples in the area of food production and food handling. The method is suitable for the enumeration of cells of E. coli that might have been subjected to stress arising from dehydration, freezing, and exposure to a saline (such as marine) environment or damage by disinfectants such as chlorine-containing products. A limitation of the applicability of this part of ISO16649 is imposed by the susceptibility of the method to a large degree of variability. The method is intended to be applied and the results interpreted in the light of the information given in Clause11. This method has not been fully evaluated for all matrices (e.g. for milk and milk products). ISO7251 is intended to be used for milk and milk products.
| Standards | Relationship |
| ISO 16649-3:2015 | Identical |
| EN ISO 16649-3:2015 | Identical |
| ISO 6887-1:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO 6887-6:2013 | Microbiology of food and animal feed — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 6: Specific rules for the preparation of samples taken at the primary production stage |
| ISO 6887-5:2010 | Microbiology of food and animal feeding stuffs Preparation of test samples, initial suspension and decimal dilutions for microbiological examination Part 5: Specific rules for the preparation of milk and milk products |
| ISO 11133:2014 | Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media |
| ISO 7251:2005 | Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of presumptive Escherichia coli — Most probable number technique |
| ISO 6887-4:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of miscellaneous products |
| ISO 6887-3:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products |
| ISO 6887-2:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 2: Specific rules for the preparation of meat and meat products |
Access your standards online with a subscription
Features
-
Simple online access to standards, technical information and regulations.
-
Critical updates of standards and customisable alerts and notifications.
-
Multi-user online standards collection: secure, flexible and cost effective.