ASTM D 6469 : 2020
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
View Superseded by
Standard Guide for Microbial Contamination in Fuels and Fuel Systems
Hardcopy , PDF
22-05-2024
English
30-09-2020
Committee |
D 02
|
DocumentType |
Guide
|
Pages |
12
|
PublisherName |
American Society for Testing and Materials
|
Status |
Superseded
|
SupersededBy | |
Supersedes |
1.1This guide provides personnel who have a limited microbiological background with an understanding of the symptoms, occurrence, and consequences of chronic microbial contamination. The guide also suggests means for detection and control of microbial contamination in fuels and fuel systems. This guide applies primarily to gasoline, aviation, boiler, industrial gas turbine, diesel, marine, furnace fuels and blend stocks (see Specifications D396, D910, D975, D1655, D2069, D2880, D3699, D4814, D6227, and D6751), and fuel systems. However, the principles discussed herein also apply generally to crude oil and all liquid petroleum fuels. ASTM Manual 472 provides a more detailed treatment of the concepts introduced in this guide; it also provides a compilation of all of the standards referenced herein that are not found in the Annual Book of ASTM Standards, Section Five on Petroleum Products and Lubricants.
1.2This guide is not a compilation of all of the concepts and terminology used by microbiologists, but it does provide a general understanding of microbial fuel contamination.
1.3The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.4This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.5This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
ASTM D 4814 : 2024 | Standard Specification for Automotive Spark-Ignition Engine Fuel |
ASTM D 7464 : 2020 | Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing |
ASTM D 8011 : 2019 | Standard Specification for Natural Gasoline as a Blendstock in Ethanol Fuel Blends or as a Denaturant for Fuel Ethanol |
ASTM D 7847 : 2022 | Standard Guide for Interlaboratory Studies for Microbiological Test Methods |
ASTM D 7960 : 2021 | Standard Specification for Unleaded Aviation Gasoline Test Fuel Containing Non-hydrocarbon Components |
ASTM D 8412 : 2021 | Standard Guide for Quantification of Microbial Contamination in Liquid Fuels and Fuel-Associated Water by Quantitative Polymerase Chain Reaction (qPCR) |
ASTM D 910 : 2024 | Standard Specification for Leaded Aviation Gasolines |
ASTM D 6227 : 2024 | Standard Specification for Unleaded Aviation Gasoline Containing a Non-hydrocarbon Component |
ASTM D 7719 : 2021 : REV A | Standard Specification for High Aromatic Content Unleaded Hydrocarbon Aviation Gasoline Test Fuel |
ASTM D 8506 : 2023 | Standard Guide for Microbial Contamination and Biodeterioration in Turbine Oils and Turbine Oil Systems |
ASTM D 7467 : 2023 | Standard Specification for Diesel Fuel Oil, Biodiesel Blend (B6 to B20) |
ASTM D 7566 : 2024 : REV A | Standard Specification for Aviation Turbine Fuel Containing Synthesized Hydrocarbons |
ASTM E 3152 : 2023 | Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents |
ASTM D 7547 : 2023 | Standard Specification for Hydrocarbon Unleaded Aviation Gasoline |
ASTM D 1655 : 2024 | Standard Specification for Aviation Turbine Fuels |
ASTM D 396 : 2024 | Standard Specification for Fuel Oils |
ASTM D 7826 : 2023 : REV B | Standard Guide for Evaluation of New Aviation Gasolines and New Aviation Gasoline Additives |
ASTM D 8434 : 2021 | Standard Specification for Unleaded Aviation Gasoline Test Fuel Containing Organo-metallic Additive |
ASTM D 7794 : 2021 | Standard Practice for Blending Mid-Level Ethanol Fuel Blends for Flexible-Fuel Vehicles with Automotive Spark-Ignition Engines |
ASTM D 7978 : 2014 : R2019 | Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water—Thixotropic Gel Culture Method |
ASTM D 6974 : 2020 | Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures |
ASTM D 975 : 2023 : REV A | Standard Specification for Diesel Fuel |
ASTM D 7544 : 2023 | Standard Specification for Pyrolysis Liquid Biofuel |
ASTM D 4057 : 2019 | Standard Practice for Manual Sampling of Petroleum and Petroleum Products |
ASTM D 4176 : 2021 | Standard Test Method for Free Water and Particulate Contamination in Distillate Fuels (Visual Inspection Procedures) |
ASTM D 3241 : 2019 | Standard Test Method for Thermal Oxidation Stability of Aviation Turbine Fuels |
ASTM D 974 : 2014 : EDT 2 | Standard Test Method for Acid and Base Number by Color-Indicator Titration |
ASTM D 974 : 2001 | Standard Test Method for Acid and Base Number by Color-Indicator Titration |
ASTM D 3241 : 2020 : REV C | Standard Test Method for Thermal Oxidation Stability of Aviation Turbine Fuels |
ASTM D 3699 : 2019 | Standard Specification for Kerosine |
ASTM D 664 : 2018 : EDT 2 | Standard Test Method for Acid Number of Petroleum Products by Potentiometric Titration |
ASTM D 1655 : 2024 | Standard Specification for Aviation Turbine Fuels |
ASTM D 3240 : 2015 | Standard Test Method for Undissolved Water In Aviation Turbine Fuels |
ASTM D 4012 : 2015 | Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water |
ASTM D 3867 : 2016 | Standard Test Methods for Nitrite-Nitrate in Water |
ASTM E 177 : 1990 : REV A : R1996 | Standard Practice for Use of the Terms Precision and Bias in ASTM Test Methods |
ASTM D 3241 : 2020 : REV B | Standard Test Method for Thermal Oxidation Stability of Aviation Turbine Fuels |
ASTM D 2068 : 2020 | Standard Test Method for Determining Filter Blocking Tendency |
ASTM D 4418 : 2022 | Standard Practice for Receipt, Storage, and Handling of Fuels for Gas Turbines |
ASTM D 2276 : 2006 : R2014 | Standard Test Method for Particulate Contaminant in Aviation Fuel by Line Sampling |
ASTM D 396 : 2020 | Standard Specification for Fuel Oils |
ASTM D 664 : 2017 : REV A | Standard Test Method for Acid Number of Petroleum Products by Potentiometric Titration |
ASTM D 4454 : 1985 : R1997 | Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy |
ASTM D 1426 : 1998 | Standard Test Methods for Ammonia Nitrogen In Water |
ASTM D 975 : 2022 | Standard Specification for Diesel Fuel |
ASTM D 7687 : 2023 | Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration |
ASTM D 1655 : 2021 : REV A | Standard Specification for Aviation Turbine Fuels |
ASTM D 7687 : 2017 | Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration |
ASTM D 7463 : 2021 | Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water |
ASTM D 4012 : 2023 : REV A | Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water |
ASTM D 5304 : 2000 | Standard Test Method for Assessing Distillate Fuel Storage Stability by Oxygen Overpressure |
ASTM D 1298 : 2012 : REV B : R2017 : EDT 1 | Standard Test Method for Density, Relative Density, or API Gravity of Crude Petroleum and Liquid Petroleum Products by Hydrometer Method |
ASTM D 396 : 1998 | Standard Specification for Fuel Oils |
ASTM D 4860 : 2022 | Standard Test Method for Free Water and Particulate Contamination in Middle Distillate Fuels (Clear and Bright Numerical Rating) |
ASTM D 4870 : 2018 | Standard Test Method for Determination of Total Sediment in Residual Fuels |
ASTM D 3867 : 2016 : R2021 : EDT 1 | Standard Test Methods for Nitrite-Nitrate in Water |
ASTM D 975 : 2019 : REV B | Standard Specification for Diesel Fuel |
ASTM D 4814 : 2024 | Standard Specification for Automotive Spark-Ignition Engine Fuel |
ASTM D 8070 : 2020 | Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay |
ASTM D 1655 : 2020 : REV D | Standard Specification for Aviation Turbine Fuels |
ASTM D 3241 : 2020 | Standard Test Method for Thermal Oxidation Stability of Aviation Turbine Fuels |
ASTM D 1655 : 2022 : REV A | Standard Specification for Aviation Turbine Fuels |
ASTM D 4412 : 2019 : R2024 | Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits |
ASTM D 974 : 2022 | Standard Test Method for Acid and Base Number by Color-Indicator Titration |
ASTM D 1298 : 2012 : REV B : R2017 | Standard Test Method for Density, Relative Density, or API Gravity of Crude Petroleum and Liquid Petroleum Products by Hydrometer Method |
ASTM D 664 : 2024 | Standard Test Method for Acid Number of Petroleum Products by Potentiometric Titration |
ASTM D 3241 : 2018 | Standard Test Method for Thermal Oxidation Stability of Aviation Turbine Fuels |
ASTM D 1655 : 2022 | Standard Specification for Aviation Turbine Fuels |
ASTM D 3241 : 2024 | Standard Test Method for Thermal Oxidation Stability of Aviation Turbine Fuels |
ASTM D 6426 : 2022 | Standard Test Method for Determining Filterability of Middle Distillate Fuel Oils |
ASTM D 6227 : 2018 | Standard Specification for Unleaded Aviation Gasoline Containing a Non-hydrocarbon Component |
ASTM D 445 : 2021 : EDT 2 | Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (and Calculation of Dynamic Viscosity) |
ASTM D 4952 : 1997 | Standard Test Method for Qualitative Analysis for Active Sulfur Species in Fuels and Solvents (Doctor Test) |
ASTM D 3240 : 2022 : REV A | Standard Test Method for Undissolved Water In Aviation Turbine Fuels |
ASTM D 975 : 2021 | Standard Specification for Diesel Fuel |
ASTM D 975 : 2023 : REV A | Standard Specification for Diesel Fuel |
ASTM D 6217 : 2021 | Standard Test Method for Particulate Contamination in Middle Distillate Fuels by Laboratory Filtration<rangeref></rangeref > |
ASTM D 974 : 2021 | Standard Test Method for Acid and Base Number by Color-Indicator Titration |
ASTM D 445 : 2017 : REV A | Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (and Calculation of Dynamic Viscosity) |
ASTM D 4814 : 2023 : REV A | Standard Specification for Automotive Spark-Ignition Engine Fuel |
ASTM D 3240 : 1991 : R1996 | Standard Test Method for Undissolved Water In Aviation Turbine Fuels |
ASTM D 3241 : 2023 | Standard Test Method for Thermal Oxidation Stability of Aviation Turbine Fuels |
ASTM D 4952 : 2023 | Standard Test Method for Qualitative Analysis for Active Sulfur Species in Fuels and Solvents (Doctor Test) |
ASTM D 6227 : 2018 : R2023 | Standard Specification for Unleaded Aviation Gasoline Containing a Non-hydrocarbon Component |
ASTM D 1976 : 1996 | Standard Test Method for Elements in Water by Inductively-Coupled Argon Plasma Atomic Emission Spectroscopy |
ASTM D 3240 : 2022 | Standard Test Method for Undissolved Water In Aviation Turbine Fuels |
ASTM D 8070 : 2019 | Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay |
ASTM D 4814 : 2022 | Standard Specification for Automotive Spark-Ignition Engine Fuel |
ASTM D 4412 : 2019 | Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits |
ASTM D 445 : 1988 | Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (and the Calculation of Dynamic Viscosity) |
ASTM D 4814 : 2021 | Standard Specification for Automotive Spark-Ignition Engine Fuel |
ASTM D 975 : 2020 : REV C | Standard Specification for Diesel Fuel |
ASTM D 3326 : 2007 : R2017 | Standard Practice for Preparation of Samples for Identification of Waterborne Oils |
ASTM D 4814 : 2021 : REV C | Standard Specification for Automotive Spark-Ignition Engine Fuel |
ASTM D 4814 : 2023 | Standard Specification for Automotive Spark-Ignition Engine Fuel |
ASTM D 515 : 1988 | Test Methods for Phosphorus in Water (Withdrawn 1997) |
ASTM D 2880 : 2023 | Standard Specification for Gas Turbine Fuel Oils |
ASTM D 2880 : 2020 | Standard Specification for Gas Turbine Fuel Oils |
ASTM D 6217 : 2018 | Standard Test Method for Particulate Contamination in Middle Distillate Fuels by Laboratory Filtration |
ASTM D 910 : 2001 | Standard Specification for Aviation Gasoline's |
ASTM D 7687 : 2021 | Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration |
ASTM D 4860 : 2021 | Standard Test Method for Free Water and Particulate Contamination in Middle Distillate Fuels (Clear and Bright Numerical Rating) |
ASTM D 1655 : 2021 : REV B | Standard Specification for Aviation Turbine Fuels |
ASTM D 664 : 1995 | Standard Test Method for Acid Number of Petroleum Products by Potentiometric Titration |
ASTM D 7463 : 2018 | Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water |
ASTM D 6426 : 2013 | Standard Test Method for Determining Filterability of Middle Distillate Fuel Oils |
ASTM D 8070 : 2021 | Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay |
ASTM D 3241 : 2023 : REV A | Standard Test Method for Thermal Oxidation Stability of Aviation Turbine Fuels |
ASTM D 1426 : 2015 : R2021 : EDT 1 | Standard Test Methods for Ammonia Nitrogen In Water |
ASTM D 4176 : 2022 | Standard Test Method for Free Water and Particulate Contamination in Distillate Fuels (Visual Inspection Procedures) |
ASTM D 910 : 2020 : REV A | Standard Specification for Leaded Aviation Gasolines |
ASTM D 1655 : 2023 | Standard Specification for Aviation Turbine Fuels |
ASTM D 910 : 2021 | Standard Specification for Leaded Aviation Gasolines |
ASTM D 910 : 2024 | Standard Specification for Leaded Aviation Gasolines |
ASTM D 1655 : 1985 : REV A | Standard Specification for Aviation Turbine Fuels |
ASTM D 445 : 2019 : REV A | Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (and Calculation of Dynamic Viscosity) |
ASTM D 4012 : 2023 | Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water |
ASTM D 6751 : 2023 : REV A | Standard Specification for Biodiesel Fuel Blendstock (B100) for Middle Distillate Fuels |
ASTM D 6426 : 2001 | Standard Test Method for Determining Filterability of Distillate Fuel Oils |
ASTM D 4418 : 1988 : R1998 | Standard Practice for Receipt, Storage, and Handling of Fuels for Gas Turbines |
ASTM D 6751 : 2020 : REV A | Standard Specification for Biodiesel Fuel Blend Stock (B100) for Middle Distillate Fuels |
ASTM D 4176 : 2021 : REV A | Standard Test Method for Free Water and Particulate Contamination in Distillate Fuels (Visual Inspection Procedures) |
ASTM D 3699 : 1998 | Standard Specification for Kerosine |
ASTM D 3241 : 2023 : REV A : EDT 1 | Standard Test Method for Thermal Oxidation Stability of Aviation Turbine Fuels |
ASTM D 5452 : 2020 | Standard Test Method for Particulate Contamination in Aviation Fuels by Laboratory Filtration |
ASTM D 4012 : 1981 : R1997 | Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water |
ASTM D 4176 : 2020 | Standard Test Method for Free Water and Particulate Contamination in Distillate Fuels (Visual Inspection Procedures) |
ASTM D 445 : 2019 | Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (and Calculation of Dynamic Viscosity) |
ASTM D 1655 : 2021 | Standard Specification for Aviation Turbine Fuels |
ASTM D 6227 : 2024 | Standard Specification for Unleaded Aviation Gasoline Containing a Non-hydrocarbon Component |
ASTM D 1655 : 2021 : REV C | Standard Specification for Aviation Turbine Fuels |
ASTM D 130 : 2018 | Standard Test Method for Corrosiveness to Copper from Petroleum Products by Copper Strip Test |
ASTM D 975 : 2022 : REV A | Standard Specification for Diesel Fuel |
ASTM D 396 : 2021 | Standard Specification for Fuel Oils |
ASTM D 3241 : 2019 : REV B | Standard Test Method for Thermal Oxidation Stability of Aviation Turbine Fuels |
ASTM D 445 : 2001 | Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (the Calculation of Dynamic Viscosity) |
ASTM D 664 : 2011 : REV A | Standard Test Method for Acid Number of Petroleum Products by Potentiometric Titration |
ASTM D 4870 : 2022 | Standard Test Method for Determination of Total Sediment in Residual Fuels |
ASTM D 6751 : 2023 | Standard Specification for Biodiesel Fuel Blend Stock (B100) for Middle Distillate Fuels |
ASTM D 396 : 2009 | Standard Specification for Fuel Oils |
ASTM D 445 : 2024 | Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (and Calculation of Dynamic Viscosity) |
ASTM D 445 : 2021 | Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (and Calculation of Dynamic Viscosity) |
ASTM D 4814 : 2021 : REV A | Standard Specification for Automotive Spark-Ignition Engine Fuel |
ASTM D 4860 : 2014 : R2019 | Standard Test Method for Free Water and Particulate Contamination in Middle Distillate Fuels (Clear and Bright Numerical Rating) |
ASTM D 1126 : 1996 | Standard Test Method for Hardness in Water |
ASTM D 4418 : 2017 | Standard Practice for Receipt, Storage, and Handling of Fuels for Gas Turbines |
ASTM D 4057 : 2022 | Standard Practice for Manual Sampling of Petroleum and Petroleum Products |
ASTM D 975 : 2023 | Standard Specification for Diesel Fuel |
ASTM D 888 : 1992 : R1996 | Standard Test Methods for Dissolved Oxygen in Water |
ASTM D 6426 : 2018 | Standard Test Method for Determining Filterability of Middle Distillate Fuel Oils |
ASTM D 445 : 2018 | Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (and Calculation of Dynamic Viscosity) |
ASTM D 3242 : 2023 | Standard Test Method for Acidity in Aviation Turbine Fuel |
ASTM D 1426 : 2015 | Standard Test Methods for Ammonia Nitrogen In Water |
ASTM D 2276 : 2022 | Standard Test Method for Particulate Contaminant in Aviation Fuel by Line Sampling |
ASTM D 1655 : 2023 : REV A | Standard Specification for Aviation Turbine Fuels |
ASTM E 1259 : 2018 | Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C |
ASTM D 4952 : 2012 : R2017 | Standard Test Method for Qualitative Analysis for Active Sulfur Species in Fuels and Solvents (Doctor Test) |
ASTM D 445 : 2021 : EDT 1 | Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (and Calculation of Dynamic Viscosity) |
ASTM E 1259 : 2023 | Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C |
ASTM D 396 : 2024 | Standard Specification for Fuel Oils |
ASTM D 3242 : 2011 : R2017 | Standard Test Method for Acidity in Aviation Turbine Fuel |
ASTM D 5452 : 2023 | Standard Test Method for Particulate Contamination in Aviation Fuels by Laboratory Filtration |
ASTM D 2068 : 2024 | Standard Test Method for Determining Filter Blocking Tendency |
ASTM D 4412 : 1984 : R1997 | Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits |
ASTM D 4814 : 2021 : REV B | Standard Specification for Automotive Spark-Ignition Engine Fuel |
ASTM D 8070 : 2023 | Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay |
ASTM D 445 : 2023 | Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (and Calculation of Dynamic Viscosity) |
ASTM D 130 : 2012 | Standard Test Method for Corrosiveness to Copper from Petroleum Products by Copper Strip Test |
ASTM D 3326 : 2007 : R2024 | Standard Practice for Preparation of Samples for Identification of Waterborne Oils |
ASTM D 1067 : 1992 : R1996 | Standard Test Methods for Acidity or Alkalinity of Water |
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