EN 12469:2000
Current
The latest, up-to-date edition.
Biotechnology - Performance criteria for microbiological safety cabinets
05-24-2000
Foreword
Introduction
1 Scope
2 Normative references
3 Definitions
4 Hazards
5 Performance classes
7 Safety requirements
8 Marking and packaging
9 Documentation
Annex A (informative) Guidance on materials, design
and manufacture
Annex B (normative) Test method for leakage of carcass
for class I and II MSCs
Annex C (normative) Test methods for the retention
efficiency at the front aperture
Annex D (informative) Aerosol challenge test method for
installed HEPA filter system
leakage detection
Annex E (normative) Test method for product
protection for class II MSCs
Annex F (normative) Test method for cross contamination
protection for class II MSCs
Annex G (informative) Method of measurement of volumetric
airflow rate
Annex H (informative) Design of MSCs and airflow velocities
in MSCs
Annex J (informative) Recommendations for decontamination,
cleaning and fumigation of MSCs and
filters
Annex K (informative) Recommendations for routine maintenance
of MSCs
Bibliography
This European Standard specifies basic requirements for microbiological safety cabinets (MSCs) with respect to safety and hygiene. This European Standard sets the minimum performance criteria for safety cabinets for work with microorganisms and specifies test procedures for microbiological safety cabinets with respect to protection of the worker and the environment, product protection and cross contamination. Mechanical, electrical, chemical or radioactive safety precautions are not covered in the standard but are covered in EN 610101-1, EN 292-1 and EN 292-2 (see Bibliography (1), (2) and (3)).
Committee |
CEN/TC 332
|
DocumentType |
Standard
|
PublisherName |
Comite Europeen de Normalisation
|
Status |
Current
|
Standards | Relationship |
BS EN 12469:2000 | Identical |
NF EN 12469 : 2000 | Identical |
I.S. EN 12469:2000 | Identical |
AS 2252.3-2011 | Identical |
GOST R EN 12469 : 2010 | Identical |
NS EN 12469 : 1ED 2000 | Identical |
NEN EN 12469 : 2000 | Identical |
SN EN 12469 : 2000 | Identical |
NBN EN 12469 : 2000 | Identical |
UNE-EN 12469:2001 | Identical |
PN EN 12469 : 2002 | Identical |
UNI EN 12469 : 2001 | Identical |
DIN EN 12469:2000-09 | Identical |
PREN 14175-3 : DRAFT 2017 | FUME CUPBOARDS - PART 3: TYPE TEST METHODS |
S.R. CWA 17253-2:2018 | JOINT IMPLANTS - PART 2: TIERED TOOLKIT APPROACH TO EVALUATE THE BIOLOGICAL IMPACT OF WEAR PARTICLES FROM JOINT REPLACEMENTS AND RELATED DEVICES |
06/30152772 DC : 0 | BS EN 15458 - PAINTS AND VARNISHES - LABORATORY METHOD FOR TESTING THE EFFICACY OF FILM PRESERVATIVES IN A COATING AGAINST ALGAE |
BS EN 15457:2014 | Paints and varnishes. Laboratory method for testing the efficacy of film preservatives in a coating against fungi |
PREN 15457 : DRAFT 2014 | PAINTS AND VARNISHES - LABORATORY METHOD FOR TESTING THE EFFICACY OF FILM PRESERVATIVES IN A COATING AGAINST FUNGI |
CSA Z15190 : 2005 : R2010 | MEDICAL LABORATORIES - REQUIREMENTS FOR SAFETY |
PD 6632:1998 | Biotechnology. Laboratories for research, development and analysis. Guidance on the selection of equipment needed for biotechnology laboratories according to the degree of hazard |
I.S. EN 15458:2014 | PAINTS AND VARNISHES - LABORATORY METHOD FOR TESTING THE EFFICACY OF FILM PRESERVATIVES IN A COATING AGAINST ALGAE |
ISO 14644-7:2004 | Cleanrooms and associated controlled environments — Part 7: Separative devices (clean air hoods, gloveboxes, isolators and mini-environments) |
EN 12128:1998 | Biotechnology - Laboratories for research, development and analysis - Containment levels of microbiology laboratories, areas of risk, localities and physical safety requirements |
EN 13312-3:2001 | Biotechnology - Performance criteria for piping and instrumentation - Part 3: Sampling and inoculation devices |
UNI EN 15457 : 2014 | PAINTS AND VARNISHES - LABORATORY METHOD FOR TESTING THE EFFICACY OF FILM PRESERVATIVES IN A COATING AGAINST FUNGI |
06/30152769 DC : 0 | BS EN 15457 - PAINTS AND VARNISHES - LABORATORY METHOD FOR TESTING THE EFFICACY OF FILM PRESERVATIVES IN A COATING AGAINST FUNGI |
17/30365068 DC : 0 | BS EN 14175-3 - FUME CUPBOARDS - PART 3: TYPE TEST METHODS |
I.S. EN 15457:2014 | PAINTS AND VARNISHES - LABORATORY METHOD FOR TESTING THE EFFICACY OF FILM PRESERVATIVES IN A COATING AGAINST FUNGI |
EN 14175-3:2003 | Fume cupboards - Part 3: Type test methods |
DIN EN 12128:1998-05 | BIOTECHNOLOGY - LABORATORIES FOR RESEARCH, DEVELOPMENT AND ANALYSIS - CONTAINMENT LEVELS OF MICROBIOLOGY LABORATORIES, AREAS OF RISK, LOCALITIES AND PHYSICAL SAFETY REQUIREMENTS |
BS EN 13312-3:2001 | Biotechnology. Performance criteria for piping and instrumentation Sampling and inoculation devices |
DIN EN ISO 7218:2014-09 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GENERAL REQUIREMENTS AND GUIDANCE FOR MICROBIOLOGICAL EXAMINATIONS (ISO 7218:2007 + AMD 1:2013, CORRECTED VERSION 2014-04-15) |
I.S. EN ISO 7218:2007 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GENERAL REQUIREMENTS AND GUIDANCE FOR MICROBIOLOGICAL EXAMINATIONS (ISO 7218:2007) |
DIN EN 14175-2:2003-08 | FUME CUPBOARDS - PART 2: SAFETY AND PERFORMANCE REQUIREMENTS |
VDI 4258 Blatt 1:2017-03 | Bioaerosols and biological agents - Generation of test bioaerosols - Basics and requirements for test bioaerosols |
VDI 6300 Blatt 1:2016-05 | Genetic engineering operations in genetic engineering facilities - Guidance on safe operation of genetic engineering facilities |
VDI 2083 Blatt 3:2005-07 | Cleanroom technology - Metrology and test methods |
VDI/VDE 3516 Blatt 2:1981-07 | Process analytical equipment for fluids |
VDI 2083 Blatt 16.1:2010-08 | Cleanroom technology - Barier systems (isolators, mini-environments, separative devices) - Effectiveness and certification |
14/30292470 DC : 0 | BS EN 15457 - PAINTS AND VARNISHES - LABORATORY METHOD FOR TESTING THE EFFICACY OF FILM PRESERVATIVES IN A COATING AGAINST FUNGI |
14/30302515 DC : 0 | BS EN 14175-3 - FUME CUPBOARDS - PART 3: TYPE TEST METHODS |
DIN EN 15457:2014-11 | Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against fungi |
PREN 14175-2 : DRAFT 2001 | FUME CUPBOARDS - PART 2: SAFETY AND PERFORMANCE REQUIREMENTS |
ISO 15190:2003 | Medical laboratories Requirements for safety |
BS EN 14175-3:2003 | Fume cupboards Type test methods |
DIN EN 15458:2014-11 | Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against algae |
BS EN 15458:2014 | Paints and varnishes. Laboratory method for testing the efficacy of film preservatives in a coating against algae |
01/122562 DC : DRAFT JULY 2001 | BS EN 14175-1 - FUME CUPBOARDS - PART 1: TERMS AND DEFINITIONS |
UNI EN 15458 : 2014 | PAINTS AND VARNISHES - LABORATORY METHOD FOR TESTING THE EFFICACY OF FILM PRESERVATIVES IN A COATING AGAINST ALGAE |
02/123878 DC : DRAFT OCT 2002 | BS EN 14175-3 - FUME CUPBOARDS - PART 3: TYPE TEST METHODS |
00/124544 DC : DRAFT NOV 2000 | BS EN 14056 - LABORATORY FURNITURE - RECOMMENDATIONS FOR DESIGN AND INSTALLATION |
DIN EN 14175-3:2004-03 | FUME CUPBOARDS - PART 3: TYPE TEST METHODS |
PREN 14175-1 : DRAFT 2001 | FUME CUPBOARDS - PART 1: TERMS AND DEFINITIONS |
BS ISO 15190:2003 | Medical laboratories. Requirements for safety |
01/122563 DC : DRAFT JULY 2001 | BS EN 14175-2 - FUME CUPBOARDS - PART 2: SAFETY AND PERFORMANCE REQUIREMENTS |
CSA Z15190 : 2005 | MEDICAL LABORATORIES - REQUIREMENTS FOR SAFETY |
PREN 15458 : DRAFT 2014 | PAINTS AND VARNISHES - LABORATORY METHOD FOR TESTING THE EFFICACY OF FILM PRESERVATIVES IN A COATING AGAINST ALGAE |
BS EN 14175-1:2003 | Fume cupboards Vocabulary |
BS EN 14056:2003 | Laboratory furniture. Recommendations for design and installation |
ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
BS EN 14175-2:2003 | Fume cupboards Safety and performance requirements |
BS EN ISO 14644-7:2004 | Cleanrooms and associated controlled environments Separative devices (clean air hoods, gloveboxes, isolators and mini-environments) |
EN 14056:2003 | Laboratory furniture - Recommendations for design and installation |
EN 14175-1:2003 | Fume cupboards - Part 1: Vocabulary |
EN ISO 14644-7:2004 | Cleanrooms and associated controlled environments - Part 7: Separative devices (clean air hoods, gloveboxes, isolators and mini-environments) (ISO 14644-7:2004) |
EN 14175-2:2003 | Fume cupboards - Part 2: Safety and performance requirements |
EN ISO 7218 : 2007 COR 2014 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GENERAL REQUIREMENTS AND GUIDANCE FOR MICROBIOLOGICAL EXAMINATIONS (ISO 7218:2007/AMD 1:2013, CORRECTED VERSION 2014-04-15) |
EN 15457:2014 | Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against fungi |
EN 15458:2014 | Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against algae |
BIS IS 16122 : 2013 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GENERAL REQUIREMENTS AND GUIDANCE FOR MICROBIOLOGICAL EXAMINATIONS |
I.S. EN 14175-3:2004 | FUME CUPBOARDS - PART 3: TYPE TEST METHODS |
14/30292467 DC : 0 | BS EN 15458 - PAINTS AND VARNISHES - LABORATORY METHOD FOR TESTING THE EFFICACY OF FILM PRESERVATIVES IN A COATING AGAINST ALGAE |
UNE-EN 15458:2014 | Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against algae |
CSA Z15190 : 2005 : R2015 | MEDICAL LABORATORIES - REQUIREMENTS FOR SAFETY |
BS EN ISO 7218 : 2007 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GENERAL REQUIREMENTS AND GUIDANCE FOR MICROBIOLOGICAL EXAMINATIONS |
DIN EN ISO 14644-7:2005-01 | Cleanrooms and associated controlled environments - Part 7: Separative devices (clean air hoods, gloveboxes, isolators and mini-environments) (ISO 14644-7:2004); German version EN ISO 14644-7:2004 |
IEST RP CC006.2 : 1997 | TESTING CLEANROOMS |
ISO 5349:1986 | Mechanical vibration — Guidelines for the measurement and the assessment of human exposure to hand-transmitted vibration |
EN 12297:1998 | Biotechnology - Equipment - Guidance on testing procedures for sterilizability |
ISO 4871:1996 | Acoustics — Declaration and verification of noise emission values of machinery and equipment |
ISO 3744:2010 | Acoustics Determination of sound power levels and sound energy levels of noise sources using sound pressure Engineering methods for an essentially free field over a reflecting plane |
EN 12298:1998 | Biotechnology - Equipment - Guidance on testing procedures for leaktightness |
VDI 2083 Blatt 3:2005-07 | Cleanroom technology - Metrology and test methods |
EN 292-1 : 1991 | SAFETY OF MACHINERY - BASIC CONCEPTS, GENERAL PRINCIPLES FOR DESIGN - BASIC TERMINOLOGY, METHODOLOGY |
EN 12741:1999 | Biotechnology - Laboratories for research, development and analysis - Guidance for biotechnology laboratory operations |
EN ISO 3744:2010 | Acoustics - Determination of sound power levels and sound energy levels of noise sources using sound pressure - Engineering methods for an essentially free field over a reflecting plane (ISO 3744:2010) |
EN 12296:1998 | Biotechnology - Equipment - Guidance on testing procedures for cleanability |
EN 13091:1999 | Biotechnology - Performance criteria for filter elements and filtration assemblies |
EN 292-2 : 91 AMD 1 95 | SAFETY OF MACHINERY - BASIC CONCEPTS, GENERAL PRINCIPLES FOR DESIGN - TECHNICAL PRINCIPLES AND SPECIFICATION |
EN 1822-1:2009 | High efficiency air filters (EPA, HEPA and ULPA) - Part 1: Classification, performance testing, marking |
EN 61010-1:2010 | Safety requirements for electrical equipment for measurement, control, and laboratory use - Part 1: General requirements |
ISO 11201:2010 | Acoustics Noise emitted by machinery and equipment Determination of emission sound pressure levels at a work station and at other specified positions in an essentially free field over a reflecting plane with negligible environmental corrections |
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