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EN 15784:2009

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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Animal feeding stuffs - Isolation and enumeration of presumptive Bacillus spp.

Superseded date

06-12-2021

Superseded by

EN 15784:2021

Published date

02-09-2009

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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Diluents and selective medium
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Precision
12 Test report
Annex A (informative) - Notes on procedure
Annex B (informative) - Results of the interlaboratory study
Bibliography

This European Standard defines general rules for the enumeration of probiotic bacilli in feeds containing bacilli (Bacillus species) as a single microorganism, component or mixed with other microorganisms. This method is not applicable to mineral feeds which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40% crude ash (Council Directive 79/373/EEC) [3].There are different categories of feed samples:a)Additives containing about 1010 colony forming units (CFU)/g;b)Premixtures containing about 108 CFU/g;c)Feeds, meal or pellets, which contain about 106 CFU/g and include complete feeding stuffs, and milk replacers.The detection limits are 500 (5 x 102) colony forming units per gram (CFU/g). The limits of determination are 2 x 104 CFU/g.

Committee
CEN/TC 327
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Superseded
SupersededBy

Standards Relationship
ONORM EN 15784 : 2009 Identical
I.S. EN 15784:2009 Identical
SN EN 15784 : 2010 Identical
NF EN 15784 : 2009 Identical
DIN EN 15784:2009-12 Identical
PN EN 15784 : 2009 Identical
BS EN 15784:2009 Identical
UNI EN 15784 : 2009 Identical
NS EN 15784 : 2009 Identical
UNE-EN 15784:2010 Identical
NEN EN 15784 : 2009 Identical
NBN EN 15784 : 2009 Identical

ISO 6887-1:2017 Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 7218:2007 Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations
EN ISO 6887-1:2017 Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017)
ISO 6497:2002 Animal feeding stuffs Sampling
ISO 6498:2012 Animal feeding stuffs — Guidelines for sample preparation
EN ISO 7218 : 2007 COR 2014 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GENERAL REQUIREMENTS AND GUIDANCE FOR MICROBIOLOGICAL EXAMINATIONS (ISO 7218:2007/AMD 1:2013, CORRECTED VERSION 2014-04-15)

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